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KMID : 0861020170320050027
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Korea Journal of Herbology
2017 Volume.32 No. 5 p.27 ~ p.38
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Suppression of colon cancer by administration of Canavalia gladiata D.C. and Arctium lappa L., Redix extracts in tumor-bearing mice model
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Jang Ji-Hye
Ji Kon-Young
Choi Hyung-Seok
Yang Won-Kyung
Kim Han-Young
Kim Kun-Hoae
Kang Hyung-Sik
Lee Young-Cheol
Kim Seung-Hyung
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Abstract
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Objective : In the present study, we examined whether Canavalia gladiata D.C. (CG) and Arctium lappa L., Redix (AL) mixture (CGAL), their components, lupeol and chicoric acid, regulate immune system and suppress the tumor in vitro and in vivo .
Methods : LPS-induced reactive oxygen species (ROS) and nitric oxide (NO) were measured after treatment with CG extract (CGE), CGAL, lupeol, chicoric acid and lupeol and chicoric acid mixture (lupeol+CA) in Raw264.7 cell. To determine the effect of CGE on immune responses, immune cell population and IgG production were assessed in mice. To investigate the effect of CGAL and their component on anti-tumor activity, tumor volume and weight were measured, cell cycles and immune cell population were analyzed in MC38 injected tumor bearing mice. Also, NK cell activity was determined in splenocyte isolated from tumor bearing mice.
Results : CGE, CGAL, lupeol, chicoric acid and lupeol+CA decreased the LPS-induced ROS and NO production without cell toxicity in RAW264.7 cells. CGE increased the immune cell populations of CD4+T, CD8+T and macrophages in various immune organ of mice. In tumor bearing mice, CGAL, lupeol, chicoric acid and lupeol+CA suppressed tumor volume and weight. In cell cycle analysis, they decreased the percentages of S phase. In addition, CGAL, lupeol, chicoric acid and lupeol+CA immune cell populations of CD4+T, CD8+Tcell, NK cell and macrophage in tumor as well as NK cell activity.
Conclusion : CGAL and its compounds may enhance immune responses and suppress tumor growth, and may be capable of developing health functional foods.
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KEYWORD
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Canavalia gladiata D.C., Arctium lappa L., Redix, Enhancement of immune response, Tumor suppression, Natural Killer
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